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19-Jan-2018 10:27

The aqueous phase was treated with RNase A (final concentration, 100 μg/ml; Fisher Scientific) for 15 min at 37°C.An equal volume of chloroform was added and roughly mixed, and the solution was centrifuged for 10 min at 12,000 × and 4°C.

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Sexual reproduction followed by meiosis and recombination plays an important role in the evolution of pathogenic fungi, by fitting populations and altering their virulence traits (7, 8).Amplification assays were carried out with a model 7500 SDS Fast system (Applied Biosystems), using a 10-μl reaction volume containing 0.2 μM (each) primers (see Table S2 in the supplemental material), 5 μl of SYBR green PCR master mix (2×), and 0.02 μl template c DNA.



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